The ad mocks the Republican for singling out the public broadcaster behind Sesame Street, PBS, for spending cuts. Romney said in a presidential debate last week that he would slash their funding, despite having a soft spot for Big Bird.
Sesame Workshop, the non-profit organisation behind the long-running children’s TV show, said that it was nonpartisan.
“We do not endorse candidates or participate in political campaigns,” a spokesperson said. “We have approved no campaign ads and, as is our general practice, have requested that the ad be taken down.”
Obama spokesman Ben LaBolt said the campaign was considering the request.
Obama has used Romney’s comments in their first debate to attack the Republican candidate’s spending priorities. Opinion polls suggest that Obama took a popularity hit after Romney’s strong debate performance.
The US elections will be held on November 6.
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