The former Beatle will round off the performance around the Queen Victoria Memorial alongside music stars from every decade of the Queen’s 60-year reign, including Tom Jones, Elton John, Shirley Bassey and Cliff Richard.
Younger acts who will play at the concert on Monday June 4, include Jessie J, JLS and singer songwriter Ed Sheeran.
The Queen and other members of the Royal Family including the Duke of Edinburgy, the Duke of Cambridge and Prince Harry, will join around 10,000 fans for the show.
Anyone who wants to attend the free concert will have to enter a national ballot online or through the post, and everyone chosen will be invited to a jubilee picnic in the Buckingham Palace garden afterwards.
The concert will be limited to 10,000 people and it will also be shown on BBC One and broadcast on BBC Radio 2.
Take That member and X Factor judge Gary Barlow, who is organising the concert, admitted it would be difficult to top Brian May’s performance at the Queen’s golden jubilee.
The Queen guitarist played an electric version of God Save the Queen from the roof of Buckingham Palace at the concert 10 years ago.
Barlow said: “We need to come up with something newer and better. Maybe we’ll fly someone in.”
Barlow has not revealed yet whether Take That will be performing at the concert.